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nikon a1 confocal microscope  (Nikon)


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    Nikon nikon a1 confocal microscope
    Nikon A1 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+nis+elements/NIS-Elements/pmc12966746-90-7-7
    Average 99 stars, based on 39694 article reviews
    nikon a1 confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Generated:

    Article Title: DYN-1 regulates SPD-2 and PLK-1 localization and mitotic spindle pole organization
    Article Snippet: .. Maximum-intensity Z-projections generated using Nikon NIS-Elements (Nikon Instruments) were used to analyze midbody defects. ..

    Article Title: DYN-1 regulates SPD-2 and PLK-1 localization and mitotic spindle pole organization
    Article Snippet: .. Maximum intensity Z-projections generated by Nikon NIS-Elements (Nikon Instruments) were used to measure cytoplasmic PLK-1::sfGFP in the anterior and posterior cytoplasm of wild-type and dyn-1(ky51) embryos. ..

    Software:

    Article Title: A spatially localised cryoinjury protocol to study liver regeneration in zebrafish
    Article Snippet: Camera system , Hamamatsu ORCA-Fusion BT sCMOS. .. Software , Nikon NIS-Elements. .. Incubation chamber , Room Temperature; not CO2 control activated.

    Article Title: Spatial patterns of microplastics in abiotic coastal matrices of the northern Oman Sea, assessed with data visualization and geostatistics.
    Article Snippet: Microplastics (MPs) are ubiquitous ecotoxic contaminants.. Assessing their level of contamination and distribution is crucial for identifying pollution sources, understanding their effects on aquatic ecosystems, and planning effective remediation measures.. Here, innovative visualization tools have been used for the first time to study patterns in MP contamination in water from littoral coastal zones, marine surface waters and sediment along the northern shorelines of the Oman Sea, stretching from the Pozm Estuary to Gwadar Bay at the Iran-Pakistan border.

    Inverted Microscopy:

    Article Title: Rnd3 regulates lung cancer cell invasion and migration independently of ROCK1 signaling via alpha 5 integrin modulation
    Article Snippet: For alpha 5 staining, cells were incubated with alpha 5 integrin antibody (150361; Abcam) for 1 h, then incubated with anti-rabbit-488 (111-545-144; Jackson Labs) plus Hoechst 33342 and phalloidin for 1 h. Coverslips were mounted onto glass slides using mounting medium (Dako). .. For epifluorescence, cells were visualized with a Nikon Ts2-FL inverted microscope equipped with a Panda sCMOS camera and images were captured with Nikon NIS-Elements. .. Actin stress fiber number quantification was measured from five fields of view (FOV) imaged using a Nikon Plan Apo Lambda D 60x oil objective; the total number of actin stress fibers per cell in five cells per FOV was counted for each condition, over at least three biological repeats.



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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence <t>microscopy</t> of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence <t>microscopy</t> of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence <t>microscopy</t> of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence <t>microscopy</t> of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Obtained images were processed and analyzed using Nikon NIS Elements software.

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software

    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan).

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software